Stat1 y701f
WebJul 14, 2016 · STAT1 WT and STAT1 Y701F plasmids were purchased from Addgene. Amplification of the human SAMHD1 full-length promoter sequence was performed as previously described 14 and was cloned into pGL3 ... WebSTAT1 STAT1 Addgene Alerts Receive email alerts when new plasmids with this gene become available. Log in to subscribe to Addgene Alerts. Description signal transducer and activator of transcription 1 Also known as CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 Species Homo sapiens Entrez ID 6772 MGC ID BC002704
Stat1 y701f
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WebJun 9, 2009 · The 35 probes regulated by both wild-type STAT1 and Y701F-STAT1 represent 30 distinct genes. More than half of these (17 of 30) function in immune responses . The functions of the other genes, including HERC6, FLJ20035, and EPSTI1, are mostly unknown. The signal for STAT1 itself was also increased in response to high STAT1 expression. WebCritical role of Syk-dependent STAT1 activation in innate antiviral immunity Author links open overlay panel Shasha Liu 1 3 , Yuan Liao 2 , Biao Chen 1 3 , Yuhai Chen 1 , Ziding Yu 2 , Haitao Wei 1 , Lianfeng Zhang 4 , Shile Huang 5 , Paul B. Rothman 6 , George Fu Gao 1 7 , Ji-Long Chen 1 2 8
WebY701F mice To investigate STAT1 activity in the absence of its tyrosine phospho- rylation, we generated Stat1 Y701F knock-in mice by targeting the gene with a construct encoding phenylalanine in position 701 (see Materials and Methods). We first examined heterozygous Stat1 Y701F/+ mice. WebFurthermore, STAT1 Y701F knockin mice showed a high susceptibility to IAV infection. These results reveal a mechanism underlying host innate immunity against viral infection. Results STAT1 is activated in a cytokine-independent manner …
WebLentiviral expression of Y701F-STAT1. Depositor. George Stark. Article. Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106. Insert. STAT1 (STAT1 Human) Use. Lentiviral Tags. FLAG . Expression. Mutation. Y701F. Promoter. CMV. Availability. Academic Institutions and … WebThe 35 probes regulated by both wild-type STAT1 and Y701F-STAT1 represent 30 distinct genes. More than half of these (17 of 30) function in immune responses (Table 1). The …
WebBMDMs of wild-type (WT), Stat1 Y701F/+ (WT/YF), or Stat1 À/À (S1) mice were treated with 5 ng/ml of IFNc for 4 and 48 h. Gene expression was measured by qPCR and normalized to Gapdh and to the...
WebFeb 12, 2016 · STAT1 is activated by Janus kinase (JAK)-mediated phosphorylation of Y701. To determine whether STAT1 contributes to cellular responses without this … file location google chromeWebISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II … file location for windows appsWebJan 19, 2024 · The antiviral response to IAV infection is also significantly suppressed in the STAT1 Y701F knockin mice. The results demonstrate that STAT1 activation is dependent … groceyasWebY701F GenBank ID M97935 Entrez Gene STAT1 ( a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91) Tag / Fusion Protein Flag (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site NotI (not destroyed) 3′ cloning site ApaI (not destroyed) 5′ sequencing primer T7 3′ sequencing primer SP6 file location hyperlinkWebOct 31, 2024 · As expected, the unphosphorylatable mutant of STAT1 Y701F failed to bind Tau(1–368) (Supplementary Fig. 4f), suggesting that the upstream tyrosine kinases that phosphorylate STAT1 may regulate ... grocery y stabbingThe previously characterised single mutations R602L and Y701F in STAT1 lead to abolished tyrosine phosphorylation and cytokine-induced nuclear accumulation [ 5, 7 ]. Therefore, these two mutations were combined in STAT1-GFP to create a construct that mimics U-STAT1 and cannot form parallel dimers with other STAT molecules. file location in jupyterWebFeb 12, 2016 · STAT1 is activated by Janus kinase (JAK)-mediated phosphorylation of Y701. To determine whether STAT1 contributes to cellular responses without this phosphorylation event, we generated mice... grocery yuba city