Cst myc antibody

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August undefined, 2024

Web1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (green), and 0.2 ug … WebMyc Antibody (9E10) is an IgG1 κ mouse monoclonal Myc antibody (also designated c-Myc antibody) that detects the Myc protein of mouse, rat, human, monkey, feline and canine origin by WB, IP, IF, IHC(P), FCM …

Myc Antibody (9E10) SCBT - Santa Cruz Biotechnology

WebGeneral description. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. [ 1] WebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous ... fix windows 10 boot issues

An ERK1/2‐driven RNA‐binding switch in nucleolin drives …

WebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. Web6x-His Tag Antibody (MA1-21315) in ICC/IF. Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature ... Webc-Myc Antibody. Applications: WB, IHC, ICC/IF, IP, ELISA. Reactivity: Human, Mouse, Rat, Tag, Feline. Publications: 749. Images: 417. ... The c-Myc protein is a 62 kDa … fix windows 10 boot with usb

Anti-Myc Tag Antibody, clone 4A6 - Sigma-Aldrich

c-Myc Antibody from Cell Signaling Technology - Biocompare

WebCells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). See ... WebCST complex. The CST complex is a cellular multiprotein complex involved in telomere maintenance. In budding yeast ( Saccharomyces cerevisiae ), it is composed of the … cannock ws11 8xpWebThe FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein. cannock woods

"WebAnti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. Research Category. Epitope Tags & General Use. Research Sub Category. " - Cst myc antibody

Cst myc antibody

Anti-HA tag antibody [HA.C5] (ab18181) Abcam

Web13 hours ago · Our previous studies demonstrated that rapamycin and rapalogs significantly induced IRES-mediated translation of cyclin D1 and c-myc mRNAs following exposure [16, 20, 21].The ability of these compounds to induce IRES activity of these two determinants was sufficient to render cells resistant to rapamycin in an AKT-dependent manner … WebApr 13, 2024 · DNA fragments were incubated overnight with specific antibody c-Myc (E5Q6W, CST, USA) and control IgG. The next day, antibody-binding magnetic beads were added. Proteinase K was used to elute and digest the immunoprecipitated product. The purified DNA product was used for subsequent PCR detection (Table S2). Mouse IgG …

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WebThe immunogen of 16286-1-AP is MYC tag Fusion Protein expressed in E. coli. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC … WebThis antibody detects c-myc protein and c-myc tagged proteins. MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and …

WebTarget Information. The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of … WebImmunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This product is out of stock now, and we suggest you go to the replacement 60003-2-Ig. Our concentration is detected with BSA as standard.

WebDec 4, 2015 · The antibody is question is the cell signaling Myc-tag antibody and I am hoping to use mouse tissues in an upcoming ChIP experiment. ... It might be more important to have a very nice anti-MYC ... WebThe c-Myc Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MYC. This antibody recognizes Human, Mouse, and Rat antigen. The c-Myc Antibody …

WebFeb 2, 2007 · DOI: 10.1002/prot.22080. Primary Citation of Related Structures: 2OR9, 2ORB. PubMed Abstract: The X-ray structure of the Fab fragment from the anti-c-myc …

WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – … can noctowl learn flyWebThe c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA … cannock ws11WebWe hypothesized that Euonymus sachalinensis (ES) induces apoptosis by inhibiting the expression of c-Myc in colon cancer cells, and this study proved that the methanol extract of ES has anticancer effects in colon cancer cells. ES belongs to the Celastraceae family and is well known for its medicinal properties. Extracts of species belonging to this family … fix windows 10 boot with flash driveWebApr 11, 2024 · Anti-CK2α Antibody: Cell Signaling: 2656S: Anti-Puromycin (12D10) antibody: Millipore: MABE343: Secondary antibodies: Rabbit IgG HRP linked: GE Healthcare: NA934: Mouse IgG HRP linked: ... before immunoprecipitation with 4 μg of anti-Myc-tag antibody (Cell Signaling) per sample, preconjugated to protein G Dynabeads … cannock ws12WebIP. ChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either MTA3 (E3X2E) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human Myc exon 2 primers and SimpleChIP ® … can noctowl learn acrobaticsWebUS11608381B2 US16/377,089 US202416377089A US11608381B2 US 11608381 B2 US11608381 B2 US 11608381B2 US 202416377089 A US202416377089 A US 202416377089A US 11608381 B2 US11608381 B2 fix window near me fix windows 10 activation error 0xc0020036